A Novel Microfluidic “cell-based” Blood Dialysis Platform for Murine Model of Sepsis

Sepsis represents an adverse systemic inflammatory response caused by microbial infection in blood. Current extracorporeal blood purifications based on removal of cytokines and endotoxin have had limited success in clinical trials which calls for alternative target removal. In this work, we demonstrate non-specific removal of bacteria , inflammatory, and other cellular components (platelets and leukocytes) from whole blood using cell margination and applied in vivo in a mouse model of sepsis. We show, for the first time, the feasibility of interfacing microfluidic device with mouse in a closed extracorporeal circuit for continuous blood filtration.